The sample 3D image of knee in Mathematica is the MRI of Markus van Almsick(https://www.youtube.com/watch?v=s1ot5MltxM8&spfreload=1).
The corresponding image dimension is {128, 128, 128}.
However, normally MRI consists of 20-25 2D slices. If this is so for the sample knee data set also, I would like to know how this image was re-sized with the above mentioned dimensions?
ImageResize
probably? I don't see why the number of images that you have would cause a problem. Did you see this question? $\endgroup$