This *is* possible, at least in version 7, but as rcollyer supposed there is a Global security option which cannot be set from within the Notebook (automatically, that is). First change the Global option: SetOptions[$FrontEnd, GlobalInitializationCellWarning -> False ] Then in the Notebook you need to evaluate: SetOptions[EvaluationNotebook[], InitializationCellEvaluation -> True, InitializationCellWarning -> False ] Or use the **Option Inspector** in either case.