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Szabolcs
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The option you are looking for is ColorFunction. When you create a color function manually using the GUI, you can press the Copy Function button at the bottom. Paste the function into the notebook, and store it in a variable named cfun. Then use Image3D[yourDataOrYourImage, ColorFunction -> cfun].

Szabolcs
  • 236.5k
  • 31
  • 641
  • 1.3k