Questions tagged [microscopy]

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2
votes
1answer
90 views

How to separate these circle based on their pixels mean intensity?

I have this image I want to separate green circles from yellow circle in two different images whiteout changing the pixel intensity information. I used the mean intensity ...
0
votes
0answers
49 views

Problem with batch processing of images

I am having some problems with a palette I have designed to apply identical image processing to microscopy images. I would like to apply identical processing across a folder of images (representing ...
1
vote
0answers
70 views

Mathematica for automatic image processing and quantification [closed]

I've been working on image analysis pipeline that involves a set of batch processing macros in ImageJ (for image segmentation) and data processing in Excel (VBA). I've got the process run in 4-5 ...
6
votes
1answer
215 views

How to track a deformable rod in video?

I have a video of nanotubes in solution taken with a confocal laser microscope. I typically track these nanotubes with Fiesta or Fiji, but in this case, there is variation in brightness that is ...
14
votes
1answer
375 views

Tracking cells as they move under a microscope

I have a video (tiff file) of cells under a microscope and I'd like to segment and track the location and sizes of them across time: It has a hundred frames: I've uploaded it here (now it is public):...