I would like to measure the density of my western blot band by measuring the area under the peak from this plot.
More specifically, my image will have several lanes with a pattern of bands in each lane (see link below). I would like to select an area and then measure the intensity of the bands as scanned from top to bottom.
I would do this for each lane by selecting same area (essentially move my rectangle over to the new lane) and then plot the density vs length of gel (down direction). I would then like to integrate the area under each curve to get a value which can then be compared to the pattern and density (increased or decreased) of the neighboring lane.
Is this possible to do in Mathematica and, if so, how can I do it. I'm very new to Mathematica and any help would greatly be appreciated.
Keywords: line, lines, spectrum, spectral