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has anybody a idea on how to detect dots centers on this kind of image. Here dots host a black and white contraste (or white and black) and are almost touching each other. This make it difficult to use ComponentMeasurements as I usualy do . Thanks for your help, Daniel

Image

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  • $\begingroup$ looks quite tricky. Maybe start by trying to correlate it with a disk-like kernel that has two distinct sides e.g: img = ColorConvert[ RemoveAlphaChannel[Import["https://i.stack.imgur.com/9RP53.png"]], "Grayscale"]; dm = DiskMatrix[15]; step = ArrayResample[{{1, -1}, {1, -1}}, Dimensions[dm], Resampling -> "Linear"]; kern1 = dm*step; MatrixPlot[kern1, ImageSize -> Small] corrk1 = ImageCorrelate[img, kern1, NormalizedSquaredEuclideanDistance]; HighlightImage[img, Binarize[corrk1]] $\endgroup$
    – flinty
    Oct 7, 2020 at 15:51
  • $\begingroup$ Notice that all dots are arranged in a hexagonal grid. If all your images have the same arrangement I would take advantage of this fact and use far more precise detection of the centers of dots - because knowing position just of two centers gives you coordinates of all others. So maybe you select manually by position of mouse pointer the two centers a the rest solve mathematically. $\endgroup$
    – azerbajdzan
    Oct 7, 2020 at 16:52

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This comes close, and I suspect a masking approach would be better given that all objects appear to be of the same size. An edge-preserving filter is applied to smooth out the background while maintaining contrast of the objects. Colors are then binned into 3 categories (high/low/background). I ignore one of the high/low, which is an area for improvement. Lastly, delete objects of an arbitrary size and count what's left.

i = Import@"https://i.stack.imgur.com/9RP53.png"
ij = ImportString[ExportString[i, "jpg"], "jpg"];
if = PeronaMalikFilter[ij,10];
data = ClusteringComponents[ColorQuantize[if, 16], 3];
ip = DeleteSmallComponents[
  Colorize[idata, ColorRules -> {1 -> Black, 2 -> Black, 3 -> Red}], 
  50]
ComponentMeasurements[ip, "Area"] //Length

enter image description here

163 objects are found.

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  • $\begingroup$ There are 9*(9 + 10) + 9=180 dots. 9+9=18 of them are on edge of the image and thus not entirely visible. Notice that the dots are arranged in hexagonal grid so I would expect the red regions to form the same hexagonal grid. $\endgroup$
    – azerbajdzan
    Oct 7, 2020 at 17:05

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