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added 231 characters in body
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kglr
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  • 18
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  • 929
Column[{Panel["Panel 1"], Panel["Panel 2"], 
  Mouseover[Panel["Panel 3"], Column@{Panel["Panel 3"], Panel["Extra Panel"]}], 
  Panel["Panel 4"]}, Spacings -> {0, 0}]

enter image description here

Or

Column[{Panel["Panel 1"], Panel["Panel 2"], 
  Dynamic@If[CurrentValue["MouseOver"], 
       Column@{Panel["Panel 3"], Panel["Extra Panel"]}, Panel["Panel 3"]], 
  Panel["Panel 4"]}, Spacings -> {0, 0}]
Column[{Panel["Panel 1"], Panel["Panel 2"], 
  Mouseover[Panel["Panel 3"], Column@{Panel["Panel 3"], Panel["Extra Panel"]}], 
  Panel["Panel 4"]}, Spacings -> {0, 0}]

enter image description here

Column[{Panel["Panel 1"], Panel["Panel 2"], 
  Mouseover[Panel["Panel 3"], Column@{Panel["Panel 3"], Panel["Extra Panel"]}], 
  Panel["Panel 4"]}, Spacings -> {0, 0}]

enter image description here

Or

Column[{Panel["Panel 1"], Panel["Panel 2"], 
  Dynamic@If[CurrentValue["MouseOver"], 
       Column@{Panel["Panel 3"], Panel["Extra Panel"]}, Panel["Panel 3"]], 
  Panel["Panel 4"]}, Spacings -> {0, 0}]
Source Link
kglr
  • 400.5k
  • 18
  • 488
  • 929

Column[{Panel["Panel 1"], Panel["Panel 2"], 
  Mouseover[Panel["Panel 3"], Column@{Panel["Panel 3"], Panel["Extra Panel"]}], 
  Panel["Panel 4"]}, Spacings -> {0, 0}]

enter image description here