This is the code I have:
\[Epsilon]s = -13.6;
\[Epsilon]so = -29.1;
\[Epsilon]p = -14.1;
ss\[Sigma] = -7.20;
sp\[Sigma] = 9.46;
\[Theta] = ((\[Pi] - \[Beta])/2);
Hmatrix0[\[Theta]_] =
{
{\[Epsilon]s, 0, ss\[Sigma], Cos[\[Theta]]*sp\[Sigma], -Sin[\[Theta]]*sp\[Sigma], 0},
{0, \[Epsilon]s, ss\[Sigma], -Cos[\[Theta]]*sp\[Sigma], -Sin[\[Theta]]*sp\[Sigma], 0},
{ss\[Sigma], ss\[Sigma], \[Epsilon]so, 0, 0, 0},
{Cos[\[Theta]]*sp\[Sigma], -Cos[\[Theta]]*sp\[Sigma], 0, \[Epsilon]p, 0, 0},
{-Sin[\[Theta]]*sp\[Sigma], -Sin[\[Theta]]*sp\[Sigma], 0, 0, \[Epsilon]p, 0},
{0, 0, 0, 0, 0, \[Epsilon]p}
}
Eigenvalues[Hmatrix0[\[Theta]]]
This is a sample of one of the eigenvalues:
Root[(38319.6 + 0. I) - 130827. Cos[[Beta]] - 116527. Cos[2 [Beta]] + (120228. - 9278.49 Cos[[Beta]] - 4004.37 Cos[2 [Beta]]) #1 + (29612. + 9.09495*10^-13 Cos[[Beta]]) #1^2 + 2480.29 #1^3 + 84.5 #1^4 + 1. #1^5 &, 1]
I wish to plot the eigenvalues as a function of beta as it ranges from $\frac{\pi}{2}$ to $\pi$ but I don't know what those hashes are and putting N[hmatrix0[$\beta$]] doesn't work.

\[Beta];Nwill work if it is numerically defined. – Mr.Wizard♦ Mar 14 at 5:36Slotin the documentation. – Verbeia♦ Mar 14 at 5:57